用途
sufficient for ~50 manual assays
IVD
for in vitro diagnostic use
储存温度
2-8°C
一般描述
dynamic range100mg/dL 1g/L
reagent volume2.5mL
sample volume200μL
temp.18 to 26°C
total test time40min
wavelength500 to 750nm
working reagent stabilityN/A
reagent volume2.5mL
sample volume200μL
temp.18 to 26°C
total test time40min
wavelength500 to 750nm
working reagent stabilityN/A
应用
比色法，终点法（Endpoint）
生化/生理作用
Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
包装
Includes:
biuret Reagent, 110 mL
Folin & Ciocalteu′s Phenol Reagent, 5 mL
Protein Standard (10 g/dL), 5 mL
biuret Reagent, 110 mL
Folin & Ciocalteu′s Phenol Reagent, 5 mL
Protein Standard (10 g/dL), 5 mL
法规信息
新产品
此项目有
P K Smith et al.
Analytical biochemistry, 150(1), 76-85 (1985-10-01)
Bicinchoninic acid, sodium salt, is a stable, water-soluble compound capable of forming an intense purple complex with cuprous ion (Cu1+) in an alkaline environment. This reagent forms the basis of an analytical method capable of monitoring cuprous ion produced in
Protein measurement with the Folin phenol reagent.
O H LOWRY et al.
The Journal of biological chemistry, 193(1), 265-275 (1951-11-01)
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