assay
≥80.0% (degree of coupling)
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 552 nm; λem 584 nm±10 nm in PBS, pH 7
storage temp.
−20°C
General description
Absorption Maximum, λmax: 231, 308, 350 nm (non-activated, PBS, pH 7.4)
552 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 66,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 574 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 650 − 670 nm
Fluorescence Quantum Yield, η: 0.37 (after photoactivation, PBS, pH 7.47)
552 nm (photoactivated, PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 66,000 M-1cm-1 (photoactivated, PBS, pH 7.4)
Fluorescence Maximum, λfl: 574 nm (photoactivated, PBS, pH 7.4)
Photoactivation Wavelength:360 - 440 nm
Recommended STED Wavelength, λSTED: 650 − 670 nm
Fluorescence Quantum Yield, η: 0.37 (after photoactivation, PBS, pH 7.47)
Application
- Abberior® CAGE 552 has been conjugated with secondary anti-guinea pig antibody for PALM (photo-activated light microscopy) imaging in MDA-MB-468 and COS-7 cells.
- It has been labelled with secondary antibody to perform dSTORM (direct stochastical optical reconstruction microscopy) for localization of deposited callose in destained Arabidopsis thaliana leaves.
- Abberior® CAGE 552 goat anti-mouse antibody has been used for FPALM (fluorescence photoactivation localization microscopy) imaging in NIH-3T3 fixed cells.
- It has been used for PALM imaging of silica colloids.
- It has also been used for PALM imaging of functionally asymmetric GPCR (G protein-coupled receptor) oligomers.
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
abberior is a registered trademark of Abberior GmbH
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable