KARPAS 299

A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin′s lymphoma in 1986.

Human Non-Hodgkin′s Ki-positive Large Cell Lymphoma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.