生物来源
hamster kidney
包装
tube of 5 μg 85011423-DNA-5UG
pkg of vial of cells 85011423-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Hamster Syrian kidney
细胞系描述
BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30μg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.
应用
Transformation and virus studies
培养基
DMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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