生物来源
human cervix
包装
tube of 5 μg 85112701-DNA-5UG
pkg of vial of cells 85112701-1VL
生长模式
Adherent
核型
2n = 55
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Transformation studies
生化/生理作用
Human cervix carcinoma
This cell line has been shown to be resistant to 8-azaguanine. The cell line was recloned in the depositor′s laboratory and is useful in transformation studies. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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法规信息
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