生物来源
rat kidney
包装
tube of 5 μg 87012902-DNA-5UG
pkg of vial of cells 87012902-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Rat kidney epithelial
细胞系描述
Cloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.
应用
NRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).
培养基
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.
其他说明
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