生物来源
mouse liver
包装
tube of 5 μg 88111403-DNA-5UG
pkg of vial of cells 88111403-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse C3H/An liver
细胞系描述
Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).
应用
Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)
培养基
NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan
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