生物来源
human brain
包装
tube of 5 μg 90021502-DNA-5UG
pkg of vial of cells 90021502-1VL
生长模式
Adherent
核型
Not specified
形态学
Astrocytoma
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human Caucasian astrocytoma
细胞系描述
Established from a Grade IV astrocytoma from a 68-year old Caucasian female. Cells exhibit strong perinuclear acid and phosphatase activity, glial fibrillary acidic protein present in 70-80% cells. HLA-DR present on approximately 20-30% cells after 48 hours culture.
应用
HLA profiles
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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