生物来源
fish ovary
包装
tube of 5 μg 90102529-DNA-5UG
pkg of vial of cells 90102529-1VL
生长模式
Adherent
核型
2n = 60
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Trout Rainbow gonad tissue
细胞系描述
From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).
应用
Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24°C. Fish cell lines detach easily during transit if the culture is very youn
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