biological source
fish ovary
packaging
tube of 5 μg 90102529-DNA-5UG, pkg of vial of cells 90102529-1VL
growth mode
Adherent
karyotype
2n = 60
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)
Biochem/physiol Actions
From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).
Trout Rainbow gonad tissue
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24°C. Fish cell lines detach easily during transit if the culture is very youn
Other Notes
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法规信息
低风险生物材料
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