生物来源
rat pancreas
包装
tube of 5 μg 93100618-DNA-5UG
pkg of vial of cells 93100618-1VL
生长模式
Semi-adherent aggregates
核型
Not specified
形态学
Pancreas cells
产品
Amylase and exocrine enzymes
受体
Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Rat exocrine pancreatic tumour
细胞系描述
Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
应用
AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex
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