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主要文件

COO原产地证书/COA分析证书

3A(tPA-30-1)

Name: 3A(tPA-30-1)
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC代码:

41106514

生物来源

human placenta

包装

tube of 5 μg 96020926-DNA-5UG
pkg of vial of cells 96020926-1VL

生长模式

Adherent

核型

Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high.

形态学

Epithelial

产品

At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase.

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

细胞系来源

Human placenta, SV40 transformed

细胞系描述

Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1

培养基

α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 36°C. Change medium 2-3 times weekly.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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