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UNSPSC Code:
41106514
Biological source:
human placenta
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high.
Morphology:
Epithelial
biological source
human placenta
packaging
tube of 5 μg 96020926-DNA-5UG, pkg of vial of cells 96020926-1VL
growth mode
Adherent
karyotype
Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high.
morphology
Epithelial
products
At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1
GMO1
Human placenta, SV40 transformed
Preparation Note
α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 36°C. Change medium 2-3 times weekly.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
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