生物来源
human cervix
包装
tube of 5 μg 96112022-DNA-5UG
pkg of vial of cells 96112022-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human cervix carcinoma
细胞系描述
HeLa DH cells are the wildtype that has been used for connexin-transfection assays and subsequent analysis of permeability and gap junction formation. The wildtype exhibits stable membrane potentials, very low gap junctional conductance and high stability under double whole-cell patch-clamp measurements. Gap junction plaques were not detected but using antibody directed against connexin 45 identified gap junction channels as dispersed particles in non typical gap junction areas.
应用
Transfection assays.
培养基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cell/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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