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D0125

Dansyl-L-alanine cyclohexylammonium salt

Name: Dansyl-<SC>L</SC>-alanine cyclohexylammonium salt
Brand: SIGMA

≥98% (TLC), suitable for protein expression

别名:

N-[[5-(Dimethylamino)-1-naphthalenyl]sulfonyl]- L-alanine cyclohexylammonium salt

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关于此项目

经验公式（希尔记法）:

C₁₅H₁₈N₂O₄S · C₆H₁₃N

化学文摘社编号:

53332-27-7

分子量:

421.55

NACRES:

NA.26

PubChem Substance ID:

329798584

UNSPSC Code:

12352209

EC Number:

258-478-3

MDL number:

MFCD00038927

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属性

产品名称

Dansyl-L-alanine cyclohexylammonium salt, fluorescent amino acid

Quality Level

200

assay

≥98% (TLC)

form

powder

technique(s)

protein expression: suitable

storage temp.

−20°C

SMILES string

NC1CCCCC1.C[C@H](NS(=O)(=O)c2cccc3c(cccc23)N(C)C)C(O)=O

InChI

1S/C15H18N2O4S.C6H13N/c1-10(15(18)19)16-22(20,21)14-9-5-6-11-12(14)7-4-8-13(11)17(2)3;7-6-4-2-1-3-5-6/h4-10,16H,1-3H3,(H,18,19);6H,1-5,7H2/t10-;/m0./s1

InChI key

CZUBQNQWSOLSJH-PPHPATTJSA-N

说明

Application

Alanine conjugated to the fluorophore 5-(dimethylamino)naphthalene-1-sulfonyl (dansyl). The unnatural amino acid has been incorporated into expressed proteins by use of a mutated aminoacyl-tRNA synthetase specific for dansylalanine.

Biochem/physiol Actions

Dansyl-L-alanine contains an N-terminal fluorophore (dansyl) making it useful as a fluorescent marker/probe. Dansylalanine can be incorporated into proteins by newly developing techniques that expand the genetic code.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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A genetically encoded fluorescent amino acid.

Summerer, D., et al.

Proceedings of the National Academy of Sciences of the USA, 108, 9785-9789 (2006)

Genetically encoding unnatural amino acids for cellular and neuronal studies.

Wenyuan Wang et al.

Nature neuroscience, 10(8), 1063-1072 (2007-07-03)

Proteins participate in various biological processes and can be harnessed to probe and control biological events selectively and reproducibly, but the genetic code limits the building block to 20 common amino acids for protein manipulation in living cells. The genetic

Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae.

Hyun Soo Lee et al.

Journal of the American Chemical Society, 131(36), 12921-12923 (2009-08-26)

Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated

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货号	GTIN
D0125-5G	04061832416038