Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Mouse IgM (μ-chain specific)-FITC antibody is specific for mouse IgM with mu chain. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM. Goat anti-mouse IgM is then conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Binds mouse IgM only; does not bind other mouse Igs.

Purified mouse IgM

Anti-Mouse IgM (μ-chain specific)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a minimum working antibody dilution of 1:128. It was also used for immunohistochemistry of rat heart tissue sections at a working dilution of 1:50. A dilution of 1:100 was used to label human ovarian-adenocarcinoma cells for immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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