packaging
pack of 15 g
manufacturer/tradename
Cytiva 17-0430-01
storage condition
(20% Ethanol )
parameter
70—140 cm/hr flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
4% agarose
matrix active group
-NH3 coupling for ligands containing primary amines
particle size
45-165 μm
average diameter
90 μm
operating pH
2-11
capacity
25-60 mg coupling capacity (alpha-chymotrypsinogen / mL resin)
separation technique
affinity
storage temp.
2-8°C
General description
CNBr-activated Sepharose™ 4B is a pre-activated media used for coupling antibodies or other large proteins containing -NH? groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
CNBr-activated Sepharose™ 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.
CNBr-activated Sepharose™ 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.
Features and Benefits
- For immobilizing antibodies or other large proteins containing -NH2 groups by coupling them to the matrix
- Over 25 years proven track record for coupling ligands containing primary amino groups
- Multipoint attachment resulting in a chemically stable product
- The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
存储类别
13 - Non Combustible Solids
法规信息
新产品
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实验方案
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Arina L Maltseva et al.
Frontiers in physiology, 5, 497-497 (2015-01-08)
Immune responses of invertebrate animals are mediated through innate mechanisms, among which production of antimicrobial peptides play an important role. Although evolutionary Polychaetes represent an interesting group closely related to a putative common ancestor of other coelomates, their immune mechanisms
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE17-0430-01 | 04061835490073 |