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Merck
CN

H3662

HEPES 钠盐 溶液

BioReagent, 1M, suitable for cell culture

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关于此项目

经验公式(希尔记法):
C8H17N2NaO4S
化学文摘社编号:
分子量:
260.29
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
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Quality Level

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase. Protease, Nickase, free, endotoxin, tested, <5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

General description

HEPES是一种不结合镁、钙、锰(II)或铜(II)离子的通用型两性离子缓冲剂。在1966年,Norman Good博士及其同事将HEPES归类为Good′s缓冲液。它在生物pH下为两性离子型,并在pH 6.8至8.2的范围内最有效。

Application

HEPES钠盐溶液适合用于细胞培养研究。它可用于制备HEPES缓冲液。它可在通透性成纤维细胞的磷酸化检测过程中用作缓冲液。它可被用于组成复杂的透析缓冲液,用于通过放射免疫检测对血清中的游离甲状腺素进行测定。


存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor



全球贸易项目编号

货号GTIN
H3662-500ML04061838354686
H3662-100ML04061833793817
H3662-1L04061833793862