H6147
HEPES
BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
别名:
4-（2-羟乙基）哌嗪-1-乙磺酸, N-（2-羟乙基）哌嗪-N′-（2-乙磺酸）
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关于此项目
经验公式（希尔记法）:
C8H18N2O4S
化学文摘社编号:
分子量:
238.30
UNSPSC Code:
12161704
NACRES:
NA.75
PubChem Substance ID:
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:
product line
BioXtra
Quality Level
assay
≥99.5% (titration)
form
powder
technique(s)
cell culture | embryo: suitable
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 500 mg/mL, clear, colorless
absorption
≤0.05 at 290 in H2O at 33%
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
HEPES被描述为生物研究领域的最佳通用缓冲液之一。在生理pH条件下，该分子是两性离子，并且在pH 6.8至8.2（pKa 7.55）范围内是一种有效的缓冲剂。HEPES已被广泛用于各种应用，包括组织培养。它通常用于缓冲与空气接触的细胞培养基。HEPES可用于Mg的 体外 实验。它通常以5 mM至30 mM的浓度用于细胞培养。
有效pH范围: 6.8 - 8.2
Application
HEPES已可用于：
- 作为改良Eagle最小必需培养基的成分，用于 体外 培养生精细胞，并作为冷冻保护剂的成分，用于保存这些生殖细胞。
- 作为改良Ham's F-10的组分，用于孵育母鹿受精卵，以实现单倍体雄性半合子受体生产
- 制备90％ Percoll溶液，用于Percoll梯度法以收集和制备精子
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
商品
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
相关内容
Product Information Sheet
HEPES Specification Comparison
Viable offspring derived from cryopreserved haploid rabbit parthenotes.
Garci'a-Ximenez F and Escriba MJ
Theriogenology, 57(4), 1319-1325 (2002)
Lilianna Szyk-Warszyńska et al.
Polymers, 11(5) (2019-05-28)
Multilayer films containing α- and β-casein and polypeptides, poly-L-lysine (PLL), and poly-L-arginine (PLArg) were formed by the layer-by-layer technique and Fourier Transform InfraRed spectroscopy with Attenuated Total Reflection (FTIR-ATR) and FTIR/Grazing Angle analyzed their infrared spectra. We investigated the changes
Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.
Aslam I and Fishel S
Human Reproduction, 13(3), 634-638 (1998)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H6147-500G | 04061833800416 |
| H6147-25G | 04061833800393 |
| H6147-100G | 04061833800379 |