usage
sufficient for 200 fluorometric tests (Blue fluorescence)
application(s)
cosmetics
food and beverages
detection method
fluorometric
storage temp.
−20°C
General description
焦磷酸 (PPi) 通过多次生化反应而生出，如 ATP 水解、DNA 和 RNA 聚合、环 AMP 形成以及脂肪酸-辅酶 a 酯的形成。其被无机焦磷酸酶水解。PPi 存在于血液和组织的细胞外基质中。
Application
焦磷酸检测试剂盒为用户提供了简单直接的微孔板分析方法，可测定多种样本中的焦磷酸水平。
Biochem/physiol Actions
通过使用独特的荧光焦磷酸传感器测定样品的焦磷酸浓度，其中焦磷酸的存在导致产生与焦磷酸成比例的荧光产物 (λex = 316/λem = 456 nm)。该检测方法比传统的基于酶的方法更简单、更耐用，是筛选酶活性或酶抑制剂的理想方法。
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F
flash_point_c
87 °C
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Kottur J and Deepak T N
Nucleic Acids Research (2018)
Peiyi Zheng et al.
Journal of molecular biology, 431(4), 764-776 (2019-01-18)
Phosphopantothenoylcysteine (PPC) synthetase (PPCS) catalyzes nucleoside triphosphate-dependent condensation reaction between 4'-phosphopantothenate (PPA) and l-cysteine to form PPC in CoA biosynthesis. The catalytic mechanism of PPCS has not been resolved yet. Coenzyme A biosynthesis protein 2 (Cab2) possesses activity of PPCS
Priyanka Kushwaha et al.
Journal of cellular physiology, 235(10), 6673-6683 (2020-01-28)
The activation of the Wnt/β-catenin signaling pathway is critical for skeletal development but surprisingly little is known about the requirements for the specific frizzled (Fzd) receptors that recognize Wnt ligands. To define the contributions of individual Fzd proteins to osteoblast
Jithesh Kottur et al.
Nucleic acids research, 46(12), 5875-5885 (2018-06-01)
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release
Tyler M Weaver et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25494-25504 (2020-10-02)
During DNA replication, replicative DNA polymerases may encounter DNA lesions, which can stall replication forks. One way to prevent replication fork stalling is through the recruitment of specialized translesion synthesis (TLS) polymerases that have evolved to incorporate nucleotides opposite DNA
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