application(s)
agriculture
detection method
colorimetric
relevant disease(s)
cancer; gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
Quality Level
General description
Glutamate dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and α-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the study of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.
Application
The kit is suitable for glutamate dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue, and culture media.)
Biochem/physiol Actions
The non-radioactive, colorimetric Glutamate Dehydrogenase (GDH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Features and Benefits
- Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
- Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
存储类别
3 - Flammable liquids
wgk
WGK 2
法规信息
含少量动物源组分生物产品
常规特殊物品
危险化学品
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