Glycerol 3-Phosphate (G3P) Assay Kit

Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway. Animals, fungi, and plants use G3P to produce ATP. It is used to regenerate NAD+ in brain and skeletal muscle cells. G3P has been linked to lipid imbalance diseases such as obesity. ​

The G3P Assay Kit uses a red substrate to quantify the concentration of G3P, which is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. The kit is an optimized "mix and read" format that is compatible with HTS applications. The limit of detection for this assay is 12.5 µM G3P in 100 µL assay volume.