Fluorimetric cADP-Ribose Assay Kit

cADP-ribose (cADPR) is a novel Ca2+ messenger derived from NAD+. ADP-riboxyl cyclase (ADPRC) catalyzes the synthesis of cADPR from NAD+, but the reaction can be reversed in the presence of high concentration of nicotinamide, producing NAD+ from cADPR stoichiometrically. The resultant NAD+ can be detected by the NAD probe. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range. The NAD+ detection using NAD probe is specific to NAD+ and has no reaction to NADH. This assay can be performed in a convenient 96-well or 384-well microplate.