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Merck
CN

N7782

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

别名:

Anti-iNOS

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rabbit

偶联物

unconjugated

抗体形式

IgG fraction of antiserum

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen 130 kDa

种属反应性

rat, mouse

技术

microarray: suitable
western blot: 1:10,000 using cells extract of murine RAW 264.7 macrophages activated with LPS and IFN-γ.

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

mouse ... Nos2(18126)
rat ... Nos2(24599)

一般描述

Anti-Nitric Oxide Synthase, inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). The NOS isoform characterized in macrophages is a 130 kDa protein, also known as macrophage NOS (macNOS; mNOS), NOS2, or inducible NOS (iNOS). iNOS is an interferon (IFN)-γ-inducible protein. This gene is located on human chromosome 17q11.

特异性

Anti-Nitric Oxide Synthase, Inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). By immunoblotting of an activated mouse macrophage RAW 264.7 cells extract, the staining of the iNOS band is specifically inhibited by the immunizing peptide.

免疫原

synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.

应用

Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

生化/生理作用

Inducible nitric oxide synthase (NOS2) produces nitric oxide (NO), which is expected to be lethal to malaria parasites in vitro.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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