usage
sufficient for 4 preparations
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
General description
GenElute HP 无内毒素质粒大量制备试剂盒可以简单、快速地从重组大肠杆菌培养物中分离不含内毒素的质粒DNA。该试剂盒采用了可快速澄清细菌裂解液的滤柱和可捕获质粒DNA的硅胶柱。配套的专利溶液可使质粒DNA结合到结合柱上，同时避免内毒素随之吸附。该技术使得用户可以维持一致的、低于 0.1 EU/μg 质粒DNA的内毒素水平。
制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
制备所得的优质、不含内毒素DNA可直接供包括内毒素敏感细胞转染等最严格的应用使用。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
Biochem/physiol Actions
通过离心分离法收集过夜的重组大肠杆菌培养液，然后通过改性SDS 碱裂解法进行处理。过滤澄清裂解液，然后加入针对无内毒素质粒制备而优化的结合溶液。随后，质粒捕获到硅胶膜上，同时避免内毒素随之吸附到膜上。通过两遍洗涤步骤，去除污染物。最后，在不含内毒素的水中洗脱结合所得的DNA。回收的质粒DNA主要以超螺旋形式存在。所含的基因组DNA和RNA均低于溴化丙啶染色琼脂糖凝胶电泳的检测水平。
Features and Benefits
- 可纯化出多达1.2 mg内毒素水平≤0.1 EU/μg高拷贝数质粒DNA
- 从沉淀细胞到获得纯化的质粒，仅需35分钟
- 方便的真空形式
Other Notes
更多信息，请见www.sigma-aldrich.com/genelutehp。
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Still not finding the right product?
Explore all of our products under GenElute™ HP 无内毒素质粒大提试剂盒
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
此项目有
相关内容
GenElute HP Plasmid Maxiprep Kit isolates endotoxin-free plasmid DNA in 35 minutes, meeting industry standards.
Paul A Beare et al.
Journal of bacteriology, 191(5), 1369-1381 (2008-12-31)
Coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human Q fever. The lack of methods to genetically manipulate C. burnetii significantly impedes the study of this organism. We describe here the cloning and characterization of
João M Furtado et al.
Investigative ophthalmology & visual science, 53(11), 6856-6862 (2012-09-07)
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules
Keith Hansen et al.
Journal of visualized experiments : JoVE, (64)(64), e3304-e3304 (2012-06-27)
Genome editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Traditional gene editing methods such as chemical-based mutagenesis or random integration of DNA sequences confer indiscriminate genetic changes in an
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| NA0400S-1KT | 04061834119043 |