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Merck
CN

P2069

Sigma-Aldrich

苯酚:氯仿:异戊醇 25:24:1，10 mM Tris（pH 8.0）、1 mM EDTA饱和

Supplied with Equilibration buffer, Molecular Biology

别名:

DNA extraction

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关于此项目

UNSPSC代码:
12190000
NACRES:
NA.52

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等级

Molecular Biology

质量水平

200

方案

≥99.0%

表单

liquid

pH值（酸碱度）

6.5-6.9(pH of phenolic phase)
7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

应用

agriculture

储存温度

2-8°C

应用

对于需要更高 pH 值的应用，例如大的完整基因组 DNA的分离，建议添加平衡缓冲液。
苯酚：氯仿：异戊醇已用于制备 RNA，用于研究从心肌活检中分离总 RNA。它还被用于在人雄激素受体（HUMARA） 克隆性检测中提取 DNA。
这批苯酚：氯仿：异戊醇 25:24:1 采用从大肠杆菌中提取 的pBR322 DNA 的粗制品进行了测试。 根据琼脂糖凝胶电泳评估，提取的 DNA 没有降解或浓度降低。 采用相同的操作程序处理纯化的 pBR322 DNA 和 λ Hind III 消化 DNA。 根据琼脂糖凝胶电泳评估，纯化的制剂没有检测到降解或浓度降低。

包装

随配 P3803 和一瓶单独的平衡缓冲液，用于将苯酚相调节至 pH 8.0 ± 0.2。

制备说明

将小瓶平衡缓冲液的全部内容物添加到大瓶苯酚：氯仿：异戊醇中。 在使用前轻柔地混合并使相实现分离，大约 2-4 小时。 这会将苯酚溶液的 pH 值从 6.7± 0.2 调整到 8.0± 0.2。

其他说明

添加平衡缓冲液后，产品可稳定 6 个月，而不添加缓冲液可稳定 2 年。 在添加平衡缓冲液后，在 -20 °C 下避光储存可将保质期延长至 1 年以上。

警示用语：

Danger

危险分类

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

靶器官

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

危险化学品
易制毒化学品（2类）

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCV7405
MKCT9360
MKCV3443
MKCT9020
MKCT9017

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. There are two layers in the bottle of Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; which layer should I use?

    The lower phase is the phase that is to be used.

  4. There is an Equilibration Buffer (Product B5658) that is included with Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1.  Is B5658 also available separately?

    No, it is only available as part of P2069.

  5. I ordered Product P2069, Phenol:Chloroform:Isoamyl Alcohol 25:24:1; I got one bottle of the Phenol:Chloroform:Isoamyl Alcohol and one bottle of B5658, Equilibration buffer. When and how am I supposed to use the B5658?

    B5658 is a Tris buffer that is used to raise the pH of the phenol:chloroform:isoamyl alcohol mixture, to make it suitable for isolation of genomic DNA. The contents of the two bottles need to be well mixed. Then allow the mixture to separate; this may take 2-4 hours. This procedure should raise the pH of the phenol phase from 6.7 to about 8.0.  Only one "treatment" is needed. This solution should be stable for up to 12 months.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. I see that you list two equilibrated Phenol:Chloroform:Isoamyl alcohol products. When should I use Product P3803, and when should I use Product P2069?

    In short, P3803 is for RNA work, and P2069 is for DNA work. Product P2069 consists of one bottle of Product P3803 and one bottle of Product B5658. The B5658 is added to the P3803 to raise the pH of the organic phase from 6.7 to 8.0; pH 8.0 is not suitable for RNA work, because RNA can degrade at the higher pH.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

Reverse Transcription Protocol

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

逆转录实验方案

将RNA逆转录成DNA的方法。使用含有逆转录酶、dNTP、引物、RNase抑制剂和缓冲液的预混试剂。快速合成cDNA。

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