biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 48 kDa
species reactivity
human
technique(s)
indirect immunofluorescence: 1-2 μg/mL using HeLa cells fixed with paraformaldehyde-Triton, western blot: 0.5-1 μg/mL using MCF7 nuclear extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RBBP4(5928)
mouse ... Rbbp4(19646)
rat ... Rbbp4(313048)
General description
Histone binding protein (RbAp48, also known as also known as RBBP4 or NURF55) contains 425 amino acids, tryptophan-aspartic acid (WD)-domain containing protein isolated as a retinoblastoma binding protein (RB1).
Immunogen
synthetic peptide corresponding to amino acids 1-14 of human RbAp48, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat.
Application
Anti-RbAp48 antibody produced in rabbit has been used in immunoprecipitation and western blot analysis. It may also be used in immunofluorescence.
Biochem/physiol Actions
RbAp48 is found in association with chromatin remodeling complexes Sin3A/histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Datasheet
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R3654-200UL | 04061835752546 |