R5028
Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse
clone 4F4, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Heterogeneous Nuclear Ribonucleoprotein-C1/C2
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关于此项目
Conjugate:
unconjugated
Clone:
4F4, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
8
Quality Level
Gene Information
human ... HNRNPC(3183)
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4F4, monoclonal
form
buffered aqueous solution
mol wt
antigen 41 kDa, antigen 43 kDa
species reactivity
monkey, human, chicken, hamster
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.1-0.2 μg/mL using HeLa cell nuclear extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-hnRNP-C1/C2 (mouse IgG1isotype) is derived from the 4F4 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with RNPs eluted from oligo (dT) cellulose column. Heterogeneous nuclear ribonucleoproteins C1/C2 (HnRNP C1/C2) are synthesized from C2 protein by alternative splicing and have an additional13-amino-acids. It is localized in nucleus and has leucine zipper-like RNA-binding motif. The hnRNP-C proteins have a single ribonucleoprotein (RNP) motif RNA-binding domain (RBD) of 80 to 100 amino acid long.
Immunogen
RNPs eluted from oligo (dT) cellulose column.
Application
Monoclonal Anti-hnRNP-C1/C2 antibody produced in mouse has been used in:
- antibody microarray
- western blotting
- enzyme linked immunosorbent assay (ELISA)`
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Heterogeneous nuclear ribonucleoproteins C1/C2(HnRNP C1/C2) is involved in protein translation, mRNA biogenesis and transport. The hnRNP-C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding. Mutation of the hnRNPC gene is implicated in embryonic lethal phenotype.
Physical form
Solution in phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Nuclear efflux of heterogeneous nuclear ribonucleoprotein C1/C2 in apoptotic cells: a novel nuclear export dependent on Rho-associated kinase activation
Lee HH, et al.
Journal of Cell Science, 117(23), 5579-5589 (2004)
A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells
Cantu C, et al.
Nucleic Acids Research, 39(2), 486-501 (2010)
The CCR4-NOT deadenylase complex controls Atg7-dependent cell death and heart function
Yamaguchi T, et al.
Science Signaling, 11(516), eaan3638-eaan3638 (2018)
hnRNP C is required for postimplantation mouse development but is dispensable for cell viability
Williamson DJ, et al.
Molecular and Cellular Biology, 20(11), 4094-4105 (2000)
Role of human heterogeneous nuclear ribonucleoprotein C1/C2 in dengue virus replication
Dechtawewat T, et al.
Virology Journal, 12(1), 14-14 (2015)
相关内容
Datasheet
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