R8529
Monoclonal Anti-RAL-A antibody produced in mouse
~2 mg/mL, clone RalA5F9.2, purified immunoglobulin, buffered aqueous solution
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Conjugate:
unconjugated
Clone:
RalA5F9.2, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
3
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RalA5F9.2, monoclonal
form
buffered aqueous solution
mol wt
antigen 25 kDa
species reactivity
monkey, mouse, human, rat
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 5-10 μg/mL using HeLa cells, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using rat brain extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RALA(5898)
mouse ... Rala(56044)
rat ... Rala(81757)
General description
Monoclonal Anti-Ral-A (mouse IgG1 isotype) is derived from the hybridoma RalA5F9.2 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. Ras-related protein Ral-A is a member of the Ras-like guanosine-5′-triphosphate (GTP) binding protein family. Ral-A protein is expressed in the islets of the pancreas, insulin-secreting cell lines and tissues during embryogenesis. The RALA gene is located on the human chromosome at 7p14.1.
Immunogen
synthetic peptide corresponding to amino-acids 189-203 of human Ral-A, conjugated to KLH.
Application
Monoclonal Anti-RAL-A antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Monoclonal Anti-Ral-A recognizes human, monkey, rat and mouse Ral-A, ~25 kDa.
Ras-related protein Ral-A can bind guanosine triphosphate (GTP) or guanosine diphosphate (GDP). It is known to possess a low intrinsic GTPase activity. Ral-A protein is involved in regulating the insulin secretion of pancreatic β-cells. This protein is also involved in membrane trafficking, oncogenic transformation and proliferation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For extended storage, freeze at –20 °C in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
E Rajcan-Separovic et al.
Human reproduction (Oxford, England), 25(11), 2913-2922 (2010-09-18)
Recurrent pregnancy loss (RPL), defined as two or more miscarriages, affects 3-5% of couples trying to establish a family. Despite extensive evaluation, no factor is identified in ∼40% of cases. In this study, we investigated the possibility that submicroscopic chromosomal
Sanda Ljubicic et al.
PloS one, 4(11), e7770-e7770 (2009-11-06)
RalA and RalB are multifuntional GTPases involved in a variety of cellular processes including proliferation, oncogenic transformation and membrane trafficking. Here we investigated the mechanisms leading to activation of Ral proteins in pancreatic beta-cells and analyzed the impact on different
D O Henry et al.
Molecular and cellular biology, 20(21), 8084-8092 (2000-10-12)
Ral GTPases have been implicated as mediators of Ras-induced signal transduction from observations that Ral-specific guanine nucleotide exchange factors associate with Ras and are activated by Ras. The cellular role of Ral family proteins is unclear, as is the contribution
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