R8903
Monoclonal Anti-hnRNP-K/J antibody produced in mouse
clone 3C2, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Heterogeneous Nuclear Ribonucleoprotein K/J
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关于此项目
Conjugate:
unconjugated
Clone:
3C2, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
5
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3C2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~60 kDa
species reactivity
hamster, Xenopus, bovine, human
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HNRNPK(3190)
General description
Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.
Immunogen
hnRNPs purified by oligo (dC) affinity chromatography.
Application
The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).
Biochem/physiol Actions
Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
hnRNP K: One protein multiple processes
Bomsztyk K, et al.
Bioessays, 26(6), 629-638 (2004)
A Proteomic Approach To Identify Candidate Substrates of Human Adenovirus E4orf6-E1B55K and Other Viral Cullin-Based E3 Ubiquitin Ligases
Dallaire F, et al.
Journal of Virology, 83(23), 12172-12184 (2009)
Heterogeneous nuclear ribonucleoprotein K upregulates the kinetochore complex component NUF2 and promotes the tumorigenicity of colon cancer cells
Sugimasa H, et al.
Biochemical and Biophysical Research Communications, 459(1), 29-35 (2015)
Cheng Zhang et al.
Arteriosclerosis, thrombosis, and vascular biology, 27(8), 1760-1767 (2007-05-05)
Inflammation is known to contribute to the pathogenesis of vascular diseases in which arterial wall extracellular matrix (ECM) homeostasis is disrupted. Tumor necrosis factor-alpha (TNF-alpha), a pivotal cytokine that regulates ECM metabolism by increasing degradation and decreasing production of arterial
SMN interacts with a novel family of hnRNP and spliceosomal proteins
Mourelatos Z, et al.
The Embo Journal, 20(19), 5443-5452 (2001)
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