SAB4200053
Anti-DNA polymerase δ p125 antibody, Rat monoclonal
clone PDG 5G1, purified from hybridoma cell culture
别名:
Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
PDG 5G1, monoclonal
Species reactivity:
human, mouse, rat
Application:
ICC, IP, WB
Citations:
4
biological source
rat
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PDG 5G1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~125 kDa
species reactivity
human, mouse, rat
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.5-1.0 μg/mL using Jurkat cells extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... POLD1(5424)
General description
Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.
Immunogen
human p125
Application
Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- co-immunoprecipitation
Biochem/physiol Actions
The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Eukaryotic DNA polymerases, a growing family
Huubscher U, et al.
Trends in Biochemical Sciences, 25(3), 143-147 (2000)
Human DNA polymerase delta is a pentameric holoenzyme with a dimeric p12 subunit
Khandagale P, et al.
Life science alliance, 2(2) (2019)
DNA Polymerase delta Is Required for Early Mammalian Embryogenesis
Uchimura A, et al.
Testing, 4(1) (2009)
Prashant Khandagale et al.
Life science alliance, 2(2) (2019-03-20)
Human DNA polymerase delta (Polδ), a holoenzyme consisting of p125, p50, p68, and p12 subunits, plays an essential role in DNA replication, repair, and recombination. Herein, using multiple physicochemical and cellular approaches, we found that the p12 protein forms a
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