Monoclonal Anti-GRAMD4 antibody produced in mouse

Monoclonal Anti-GRAMD4 (mouse IgG1 isotype) is derived from the hybridoma GR4-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. GRAM domain containing 4 (GRAMD4) is localized in the nucleus and is mapped to human chromosome 22q13.31.

synthetic peptide corresponding to a sequence at the N-terminal region of human GRAMD4

Monoclonal Anti-GRAMD4 antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• flow cytometry

GRAM domain containing 4 (GRAMD4) also known as DIP (Death-inducing protein), is a novel p53- independent pro-apoptotic target of E2F Transcription Factor 1 (E2F1), which localizes to the nucleus. Upregulation of GRAMD4 by E2F1 mediates apoptosis in karyotypically normal fibroblasts and tumor cells. GRAMD4 translocation to the mitochondria is mediated by p73- induced apoptosis. It interacts with B-cell lymphoma 2 (Bcl-2), promotes BCL2 associated X, apoptosis regulator (Bax) mitochondrial relocalization and oligomerization. It induces mitochondrial membrane permeabilization mediating the release of cytochrome c and second mitochondria-derived activator of caspases (Smac). Interestingly, GRAMD4 overexpression inhibits tumor growth. Its efficacy was more pronounced over time when combined with cisplatin, leading to prolonged growth retardation.

Monoclonal Anti-GRAMD4 recognizes human and monkey GRAMD4.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For extended storage, freeze at -20 °Cin working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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