SAB4200676
Monoclonal Anti-SPAG11 antibody produced in mouse
clone 10B4, purified from hybridoma cell culture
别名:
Anti-Spag11, Anti-Spag11a/b, Bin1b, sperm associated antigen 11A/B
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关于此项目
NACRES:
NA.41
UNSPSC Code:
51111800
Clone:
10B4, monoclonal
Species reactivity:
rat, mouse
Application:
IHC
Citations:
4
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10B4, monoclonal
form
buffered aqueous solution
mol wt
monomer ~7 kDa, ~34 kDa (multimer)
species reactivity
rat, mouse
concentration
~1 mg/mL
technique(s)
immunoblotting: 4-8 μg/mL using whole extracts of HEK-293 cells overexpressing rat SPAG11, immunohistochemistry: 5-10 μg/mL using paraffin-embedded rat caput epididimys sections
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Spag11a(78128)
rat ... Spag11a(246305)
General description
Monoclonal Anti-SPAG11 (mouse IgG1 isotype) is derived from the hybridoma 10B4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. SPAG11 is expressed specifically in epithelial cells in the middle part of the caput region. It exists in four isoforms such as A, D, E and G.
SPAG11A (sperm associated antigen 11A) belongs to the β defensin family. The protein is mainly expressed in the epididymis. The gene is mapped to human chromosome 8.
Immunogen
a recombinant rat SPAG11
Application
Monoclonal Anti-SPAG11 antibody produced in mouse may be used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Monoclonal Anti-SPAG11 recognizes mouse and rat SPAG11.
SPAG11 is involved in promoting sperm motility due to its capability to bind the sperm head in different regions of the epididymis. It exhibits anti-microbial activity by suppressing colony growth of Escherichia coli and other Gram-negative bacteria.
SPAG11A (sperm associated antigen 11A) is a secretory protein with possible involvement in sperm maturation. It also controls inflammatory responses in the epididymis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezer is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Identification, cloning and functional characterization of novel sperm associated antigen 11 (SPAG11) isoforms in the rat
Yenugu S, et al.
Reproductive Biology and Endocrinology, 4(1), 1-14 (2006)
Dwi A Pujianto et al.
Reproductive biology and endocrinology : RB&E, 11, 59-59 (2013-07-03)
Epididymal sperm maturation occurs via interactions between sperm and proteins secreted by the epididymal epithelium. Although this is an important process, the genes that encode the involved proteins remain largely uncharacterized. Previous studies have demonstrated that the genes involved in
Multimerin-2 maintains vascular stability and permeability
Pellicani R, et al.
Matrix Biology, 87, 11-25 (2020)
Z Fei et al.
Genes and immunity, 13(6), 445-451 (2012-04-27)
The mBin1b is a beta-defensin gene identified in the mouse epididymis. In the current report, its expression pattern and antibacterial activities were characterized, and a transgenic (TG) mouse model was developed in which mBin1b was exclusively overexpressed by up to
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