SAB4200708
Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal
clone PC 10, purified from hybridoma cell culture
别名:
Anti-ATLD2, Anti-Cyclin, Anti-PCNA
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
PC 10, monoclonal
Application:
ELISA, FACS, IF, IHC, IP
Citations:
9
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PC 10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~36 kDa
species reactivity
monkey, rat, insect, mouse, yeast, human
concentration
~1.0 mg/mL
technique(s)
ELISA: suitable, flow cytometry: suitable, immunoblotting: 0.5-1 μg/mL using human foreskin fibroblast HS-68 cell line extract, immunofluorescence: 1.25-2.5 μg/mL using HeLa cells, immunohistochemistry: 10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human Tonsil sections., immunoprecipitation (IP): suitable
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PCNA(5111)
General description
Anti-Proliferating Cell Nuclear Antigen antibody (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PCNA is an auxiliary protein of DNA polymerase δ and belongs to the conserved sliding clamp family of proteins.
PCNA (proliferating cell nuclear antigen) gene is mapped in human chromosome 20p12.3. PCNA is a homotrimeric protein, which is expressed in the nucleus. The PCNA ring has a large central cavity that allows double-stranded DNA to slide freely along it.
Immunogen
PCNA-Protein A fusion protein.
Application
Anti-Proliferating Cell Nuclear Antigen antibody, Mouse monoclonal may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- flow cytometry
- immunohistochemistry
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Monoclonal Anti-Proliferating Cell Nuclear Antigen (PCNA) specifically recognizes Proliferating Cell Nuclear Antigen from human, monkey, rat, mouse, insect and yeast origin.
PCNA (proliferating cell nuclear antigen) maintains the polymerase and DNA association by forming a sliding clamp and ensures efficient DNA replication. PCNA is responsible for recruiting important regulatory factors for DNA damage tolerance mechanism. In response to UV irradiation-induced DNA damage, monoubiquitination of PCNA mediates the polymerase switching model by recruiting DNA polymerase η to PCNA. The protein is involved in base excision repair and maintains genome stability. PCNA is associated with proteolysis coupled to DNA synthesis and repair for proper cell cycle progression.
PCNA protein is essential for chromosomal DNA replication during S-phase and is required for several DNA processes like DNA repair, sister chromatid cohesion and cell cycle control. This protein is also involved in the epigenetic modification and chromatin assembly and remodeling. PCNA acts as a prognostic and diagnostic marker in several diseases such as breast cancer, chronic lymphoid leukemia, non-Hodgkin′s lymphoma, malignant and nonmalignant skin diseases and systemic lupus erythematosus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
The Functions of Serine 687 Phosphorylation of Human DNA Polymerase ? in UV Damage Tolerance.
Dai X
Molecular and Cellular Proteomics (2016)
PCNA-Dependent Cleavage and Degradation of SDE2 Regulates Response to Replication Stress.
Jo U
PLoS Genetics (2016)
Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.
Cobb AM
Nucleus (Austin, Tex.) (2016)
Replication Protein A Prohibits Diffusion of the PCNA Sliding Clamp along Single-Stranded DNA.
Hedglin M
Biochemistry (2017)
Shao-Chun Wang
Trends in pharmacological sciences, 35(4), 178-186 (2014-03-25)
Proliferating cell nuclear antigen (PCNA) is known as a molecular marker for proliferation given its role in replication. Three identical molecules of PCNA form a molecular sliding clamp around the DNA double helix. This provides an essential platform on which
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