SAB4200774
Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal
clone 13C4, purified from hybridoma cell culture
别名:
Anti-SLT-1 B subunit, Anti-SLT-1b, Anti-Shiga-like toxin 1 subunit B, Anti-Verocytotoxin 1 subunit B, Anti-Verotoxin 1 subunit B, Anti-stxB
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关于此项目
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
13C4, monoclonal
Application:
FACS, IP
Citations:
4
isotype
IgG1
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
13C4, monoclonal
description
Research area: Microbiome
form
buffered aqueous solution
mol wt
~7 kDa
species reactivity
E. coli
concentration
~1.0 mg/mL
technique(s)
flow cytometry: 2.5-5 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit, immunoblotting: 1-2 μg/mL using purified recombinant Shiga Toxin 1-B subunit produced in E. coli (using PVDF membrane), immunoprecipitation (IP): suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Monoclonal Anti-Shiga Toxin 1, B Subunit (mouse IgG1 isotype) is derived from the 13C4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a purified Shiga-like toxin from E. coli H30. Shiga toxins consist of two polypeptides, an A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin.
The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. The latter may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications.
Immunogen
purified Shiga-like toxin from E.coli H30
Application
Anti-Shiga Toxin 1, B Subunit (STxB) antibody, Mouse monoclonal has been used in:
- immunoblotting
- flow cytometry
- immunoprecipitation
Biochem/physiol Actions
Monoclonal Anti-Shiga Toxin 1, B Subunit antibody specifically recognizes the B subunit of Shiga holotoxin.
Shiga-toxin (Stx) produced by E. coli O157:H7 may cause bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. All the Stx isoforms have similar structure and mechanism of action. The catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Gala(1-4)-Galb (1-4)-Glcb1-ceramide]. Gb3 is overexpressed in membranes of numerous tumor cells, Therefore, STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Shiga toxin: purification, structure, and function
Donohue-Rolfe A, et al.
Reviews of Infectious Diseases, 13, S293-S297 (1991)
Human colorectal tumors and metastases express Gb3 and can be targeted by an intestinal pathogen-based delivery tool
Falguieres T, et al.
Molecular Cancer Therapeutics, 7(8), 2498-2508 (2008)
In vivo tumor targeting by the B-subunit of shiga toxin
Viel T, et al.
Molecular Imaging, 7(6), 7290-2008 (2008)
Shiga toxin and its use in targeted cancer therapy and imaging
Engedal N, et al.
Microbial Biotechnology, 4(1), 32-46 (2011)
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