biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
20.0-30.0 ng/mL ED50
mol wt
9.7 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NRN1(51299)
General description
NRN1 (neuritin 1) is a highly conserved extracellular GPI (glycosylphosphatidylinositol)-anchored protein that was initially identified in rat hippocampal dentate gyrus in a screening of candidate kainic acid-induced plasticity related genes. This protein is also secreted in a soluble form.
Recombinant human Neuritin is a covalently disulfide-linked homodimer, consisting of two 9.8kDa polypeptide monomers, each containing 89 amino acid residues.
Recombinant human Neuritin is a covalently disulfide-linked homodimer, consisting of two 9.8kDa polypeptide monomers, each containing 89 amino acid residues.
Biochem/physiol Actions
NRN1 (neuritin 1) is implicated in synaptic plasticity during brain development, and is an activity-dependent protein. Synaptic plasticity is dependent upon neuronal migration, and a study shows that NRN1 protein facilitates neuronal cell migration, and its levels influence microtubule stability. This protein also promotes dendritic arborization and synapse maturation in the central nervous system (CNS). It facilitates the survival of neural progenitors and differentiated neurons during early embryonic development, and during late development it is responsible for the maturation and formation of synapses by promoting the growth and stabilization of axons and dendrites. Study in human microendothelial cells (HMEC-1) shows that the expression of this protein is hypoxia-induced, and it is a hypoxic perinecrotic marker.
Recombinant human Neuritin is a covalently disulfide-linked homodimer, consisting of two 9.8kDa polypeptide monomers, each containing 89 amino acid residues.
Recombinant human Neuritin is a covalently disulfide-linked homodimer, consisting of two 9.8kDa polypeptide monomers, each containing 89 amino acid residues.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MAGKCDAVFK GFSDCLLKLG DSMANYPQGL DDKTNIKTVC TYWEDFHSCT VTALTDCQEG AKDMWDKLRK ESKNLNIQGS LFELCGSGN
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Characterization of the expression of the hypoxia-induced genes neuritin, TXNIP and IGFBP3 in cancer.
Le Jan S et al
Febs Letters, 580(14), 3395-3400 (2006)
Neuritin 1 promotes neuronal migration.
Zito A et al
Brain Structure &Amp; Function, 219(1), 105-118 (2014)
Neuritin 1 promotes retinal ganglion cell survival and axonal regeneration following optic nerve crush.
Sharma TP et al
Cell Death & Disease, 6, e1661-e1661 (2015)
G S Naeve et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(6), 2648-2653 (1997-03-18)
Neural activity and neurotrophins induce synaptic remodeling in part by altering gene expression. A cDNA encoding a glycosylphoshatidylinositol-anchored protein was identified by screening for hippocampal genes that are induced by neural activity. This molecule, named neuritin, is expressed in postmitotic-differentiating
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