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Merck
CN

T4661

Trizma ® 碱

≥99.9% (titration), crystalline

别名:

2-氨基-2-(羟甲基)-1,3-丙二醇, THAM, Tris 碱, 三羟甲基氨基甲烷, 氨基丁三醇

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关于此项目

线性分子式:
NH2C(CH2OH)3
化学文摘社编号:
77-86-1
分子量:
121.14
UNSPSC Code:
12352104
NACRES:
NA.25
PubChem Substance ID:
24900061
EC Number:
201-064-4
Beilstein/REAXYS Number:
741883
MDL number:
MFCD00004679

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InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO

description

aminopeptidase substrate

assay

≥99.9% (titration)

form

crystalline

loss

≤5% (loss on drying, 110 °C)

color

white

pH

10.0-11.5

useful pH range

7-9

Quality Level

400

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: soluble (400 g plus 600 mL of water)

λ

(290nm < 0.05), in 5 mM Tris; DNA (40% (w/w))

application(s)

diagnostic assay manufacturing

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相关类别

General description

Tris是用于生物化学和分子生物学公认的基础标准品和缓冲液。它可以单独用作缓冲液或混合缓冲液配方的组分，如Tris-EDTA（TE）缓冲液、Tris-乙酸盐-EDTA（TAE）缓冲液、Tris-硼酸盐-EDTA（TBE）缓冲液等。 它具有高纯度、较稳定、相对不吸湿、并且具有高的当量。

Application

Tris base已被用于：
  • 作为细胞破裂的裂解缓冲液的组分
  • 制备用于PAGE（聚丙烯酰胺凝胶电泳）的TBE溶液
  • 肽核酸（PNA）的双链复合物及其互补DNA序列的研究中（通过使用阴离子交换HPLC）
  • 毛细管电色谱和生育酚和生育三烯酚的紫外分析中

Features and Benefits

Features:
  1. Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
  2. Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
  3. M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
  4. Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
  5. Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
  6. Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
    1. Site quality assessment
    2. ISO 9001 certificate
    3. Detailed product specifications
    4. Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
Benefits:
  1. Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
  2. Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
  3. Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
  4. Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
  5. Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
  6. Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.

Packaging

所有规格与 T1503 相同。在生物技术方面，用于诊断或制造。请咨询最小起订量。

Analysis Note

未经过热原测试。可提供包含批次特定信息的分析证书。

Other Notes

所有缓冲液的 pH 值都取决于温度和浓度。Tris 缓冲液，温度每降低 1°C，pH 值增加约 0.03 个单位，每稀释十倍 pH 值降低0.03-0.05 个单位。
对于精确的应用，使用经过精心校准的 pH 计和玻璃/甘汞组合电极。

Legal Information

M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000525652
0000525652
0000486945
0000486945
0000482506

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Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris.
Tear CJY, et al.
Microbial cell factories, 12(1), 106-106 (2013)
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells.
Caglayan M, et al.
Bio-protocol, 5(11) (2015)
Genome-wide mapping of embedded ribonucleotides and other noncanonical nucleotides using emRiboSeq and EndoSeq
Ding J, et al.
Nature Protocols, 10(9), 1433-1433 (2015)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)
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