T5327
Anti-TTP (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-G0/G1 switch regulatory protein 24, Anti-GOS24, Anti-Growth factor-inducible nuclear protein NUP475, Anti-NUP475, Anti-RNF162A, Anti-TIS11, Anti-Tristetraprolin, Anti-Zfp36, Zinc finger protein 36 homolog Protein
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
30
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours, western blot: 0.5-1.0 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ZFP36(7538)
mouse ... Zfp36(22695)
rat ... Zfp36(79426)
General description
TTP (N-terminal) (also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. TTP belongs to a family of human
TTP belongs to a family of human ARE (AU-rich element found in 3′ UTR mRNA) binding protein TTP mRNA is widely distributed among tissue types, with a higher expression level in spleen, lymph nodes, and thymus.
Tristetraprolin (TTP), also known as zinc-finger protein 36 (ZFP36), is a RNA-binding protein, encoded by the gene mapped to human chromosome 19q13.2. TTP is ubiquitously expressed and is characterized with a highly homologous RNA binding domain (RBD) with two CCCH zinc fingers (ZFs).
Immunogen
synthetic peptide corresponding to amino acids 51-67 of human TTP, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
Application
TTP (N-terminal) antibody produced in rabbit has been used in following studies:
- RNA EMSA supershift
- immunoblotting
- ribonucleoprotein immunoprecipitation (RNP-IP)
Biochem/physiol Actions
In activated monocytes and T lymphocytes, it regulates the expression of tumor necrosis factor a (TNF- α) by binding to a conserved AU rich element within the 3′ UTR of TNF-α mRNA. TTP is a phosphoprotein reported to be a substrate for kinases like MAPK activated protein kinase 2 (MAPKAPK2). The p38 signal transduction pathway is required for the expression of TTP protein and mRNA.
Tristetraprolin (TTP) plays a key role in maintaining the stability of certain adenosine/uridine AU-rich element (ARE) mRNAs, by removing of the poly(A) tail and enhancing mRNA turnover. The encoded protein participates in mRNA binding and destabilization.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis.
Moniaux N
Oncogene, 25, 3247-3257 (2006)
A pathogenetic role for TNFalpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency
Taylor GA, et al.
Immunity, 4(5), 445-454 (1996)
The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis
Carrick DM, et al.
Arthritis Research & Therapy, 6(6), 248-248 (2004)
Yingqing Chen et al.
Oxidative medicine and cellular longevity, 2017, 9427583-9427583 (2017-06-14)
Pterostilbene, a dimethyl ester analog of resveratrol, has anti-inflammatory and antioxidative effects and alters cell proliferation. Tristetraprolin (TTP) promotes the degradation of proinflammatory mediators via binding to adenosine and uridine- (AU-) rich elements (ARE) located in the 3'-untranslated regions of
ELAVL1 Modulates Transcriptome-wide miRNA Binding in Murine Macrophages
Lu YC
Cell Reports, 9, 2330-2343 (2014)
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