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T9767

Trypsin inhibitor

Name: Trypsin inhibitor
Brand: SIGMA

powder, suitable for electrophoresis

别名:

SBTI

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关于此项目

化学文摘社编号:

9035-81-8

UNSPSC Code:

12352200

NACRES:

NA.77

EC Number:

232-906-9

MDL number:

MFCD00082103

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属性

产品名称

胰蛋白酶抑制剂来源于大豆, BioReagent, For use as a marker in SDS-PAGE

biological source

Glycine max (soybean)

Quality Level

200

product line

BioReagent

form

powder

mol wt

20,000 Da

packaging

vial of 5 mg

technique(s)

electrophoresis: suitable

solubility

balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble

shipped in

ambient

storage temp.

2-8°C

说明

Application

来源于大豆（黄豆）的胰蛋白酶抑制剂用于电泳中标准分子量标记。

Biochem/physiol Actions

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X，机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物，然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

Preparation Note

胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊，呈黄色至琥珀色。使用胰蛋白酶消化细胞后，重悬于胰蛋白酶抑制剂溶液中，使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心，得到细胞沉淀。

在2-8℃下短期储存时，溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

Other Notes

一个胰蛋白酶单位是指在25°C、pH 7.6条件下，以N-α-苯甲酰基-L-精氨酸乙酯（BAEE）为底物，每分钟产生0.001的 A₂₅₃所需的酶量。

大豆胰蛋白酶抑制剂是一种单体蛋白，在两个二硫键交联的单个多肽链中含有181个氨基酸残基。

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pictograms

GHS08

signalword

Danger

hcodes

H317 - H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

0000456004

0000444874

0000350178

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实验方案

Trypsin Inhibitors

Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.

胰蛋白酶抑制剂

天然胰蛋白酶抑制剂也称为丝氨酸蛋白酶抑制剂（serpins），是最大和最多样化的蛋白酶抑制剂家族。Serpins通过抑制体内丝氨酸蛋白酶来控制蛋白质的活化和分解代谢。

商品

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

Endosymbiotic and host proteases in the digestive tract of the invasive snail Pomacea canaliculata: diversity, origin and characterization

Godoy MS, et al.

Testing, 8(6), e66689-e66689 (2013)

Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)-Fluorescence Analysis of Proteins.

Isabelle Le Potier et al.

Methods in molecular biology (Clifton, N.J.), 1466, 1-10 (2016-07-31)

Capillary electrophoresis (CE) coupled to fluorescence detection is an invaluable technique for the quantitative analysis of proteins of interest in the field of clinical diagnosis and quality control of novel biotechnology products. The various chemical and instrumental approaches that have

Endosymbiotic and host proteases in the digestive tract of the invasive snail Pomacea canaliculata: diversity, origin and characterization.

Martín S Godoy et al.

PloS one, 8(6), e66689-e66689 (2013-07-03)

Digestive proteases of the digestive tract of the apple snail Pomacea canaliculata were studied. Luminal protease activity was found in the crop, the style sac and the coiled gut and was significantly higher in the coiled gut. Several protease bands

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全球贸易项目编号

货号	GTIN
T9767-1VL	04061837390463