T9784
三(羟甲基)甲基甘氨酸
BioXtra, pH 4.0-6.0 (1 M in H2O), ≥99% (titration)
别名:
N-三(羟甲基)甲基甘氨酸
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关于此项目
线性分子式:
(HOCH2)3CNHCH2CO2H
化学文摘社编号:
分子量:
179.17
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
227-193-6
Beilstein/REAXYS Number:
1937804
MDL number:
product line
BioXtra
Quality Level
assay
≥99% (titration)
form
powder
impurities
≤0.005% Phosphorus (P), ≤0.1% Insoluble matter
ign. residue
≤0.1%
pH
4.0-6.0 (1 M in H2O)
useful pH range
7.4-8.8
pKa (25 °C)
8.1
solubility
H2O: 1 M, clear, colorless
anion traces
chloride (Cl-): ≤0.1%, sulfate (SO42-): ≤0.05%
cation traces
Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
absorption
≤0.05 at 280 in H2O at 1 M, ≤0.1 at 260 in H2O at 1 M
application(s)
diagnostic assay manufacturing
SMILES string
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
General description
Tricine 这个名字来源于“tris”和“glycine”。在辐射诱导的膜损伤研究中，Tricine 和其他良好的缓冲剂是羟基自由基的有效清除剂。Tricine 被广泛用作电泳缓冲液和细胞颗粒的再悬浮。由于与甘氨酸相比，它的负电荷较低，因此迁移速度较快。它具有高离子强度，这导致更多的离子运动和更少的蛋白质运动。这有助于在较低百分比的丙烯酰胺凝胶中分离小分子量蛋白质。Tricine 也被用作尾随离子，与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）系统相比，它可以在更低的丙烯酰胺浓度下分离小分子蛋白质。
Application
Tricine 已用于：
- 单细胞采集的聚合酶链式反应（PCR）缓冲液
- 作为泌乳素补充培养基的一种组分，用于培养乳泌乳素
- 制备萤光素酶测定试剂，用于萤火虫（萤火虫）中的萤光素酶活性测定，并作为 Triton/甘氨酰甘氨酸裂解缓冲液组分
用于分离低分子量的肽的缓冲剂组分。
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
相关内容
Product Information Sheet
Encyclopedia of Dairy Sciences (2011)
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T9784-250G | 04061837396052 |
| T9784-100G | 04061837391569 |
| T9784-25G | 04061833625125 |
| T9784-1KG | 04061826720448 |