表单
powder
比活
≥10,000 units/g solid
储存温度
2-8°C
一般描述
藻酸盐裂解酶存在于几种海藻、海洋软体动物以及多种海洋和陆地细菌中。
应用
藻酸盐裂解酶可用于分解藻酸盐或海藻酸，也可用于降低粘度。可用于卵泡分离、包封和培养过程中。
藻酸盐裂解酶已被用于：
- 酶的预处理
- 去封装细胞
- 消化假单胞菌外多糖，以促进电穿孔
生化/生理作用
可将藻酸盐或海藻酸分解成小分子并降低粘度。
藻酸盐裂解酶可积极参与酸性多糖的糖化，以生产生物乙醇。
制备说明
将0.15 ml酶溶液（1 un/ml）加到4.5 ml 0.1％藻酸钠（pH 6.3）中。 37℃孵育30分钟。加入4.65 ml 0.1 N NaOH可终止反应。
其他说明
在37℃、pH 6.3条件下，一单位酶可使每毫升藻酸钠溶液的A235nm每分钟增加1.0
想查看有关 复合碳水化合物分析酶 的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
François Thomas et al.
Environmental microbiology, 14(9), 2379-2394 (2012-04-20)
Alginate constitutes a significant part of seaweed biomass and thus a crucial nutrient for numerous marine heterotrophic bacteria. However, the mechanisms for alginate assimilation remain largely unknown in marine microorganisms. We show here that the genome of the marine flavobacterium
Bunzo Mikami et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 9), 1207-1216 (2012-09-06)
The structures of two mutants (H192A and Y246F) of a mannuronate-specific alginate lyase, A1-III, from Sphingomonas species A1 complexed with a tetrasaccharide substrate [4-deoxy-L-erythro-hex-4-ene-pyranosyluronate-(mannuronate)(2)-mannuronic acid] were determined by X-ray crystallography at around 2.2 Å resolution together with the apo form
Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications
Zhu B and Yin H
Bioengineered, 6(3), 125-131 (2015)
Solaleh Khoramian Tusi et al.
Biomaterials, 32(23), 5438-5458 (2011-05-06)
Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. We sought to elucidate possible effects of alginate oligosaccharide (AOS) on H(2)O(2)-induced cell death and to determine the underlying molecular mechanisms in neuron-like PC12 cells. We found
Yuya Morimoto et al.
APL bioengineering, 4(2), 026101-026101 (2020-04-09)
Biohybrid robots composed of biological and synthetic components have been introduced to reconstruct biological functions in mechanical systems and obtain better understanding of biological designs. For example, biohybrid robots powered by skeletal muscle tissue have already succeeded in performing various
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