产品名称
藻酸盐裂解酶, powder, ≥10,000 units/g solid
form
powder
specific activity
≥10,000 units/g solid
storage temp.
2-8°C
Quality Level
Preparation Note
将0.15 ml酶溶液(1 un/ml)加到4.5 ml 0.1%藻酸钠(pH 6.3)中。 37℃孵育30分钟。加入4.65 ml 0.1 N NaOH可终止反应。
Application
藻酸盐裂解酶可用于分解藻酸盐或海藻酸,也可用于降低粘度。可用于卵泡分离、包封和培养过程中。
藻酸盐裂解酶已被用于:
- 酶的预处理
- 去封装细胞
- 消化假单胞菌外多糖,以促进电穿孔
Biochem/physiol Actions
可将藻酸盐或海藻酸分解成小分子并降低粘度。
藻酸盐裂解酶可积极参与酸性多糖的糖化,以生产生物乙醇。
General description
藻酸盐裂解酶存在于几种海藻、海洋软体动物以及多种海洋和陆地细菌中。
Other Notes
在37℃、pH 6.3条件下,一单位酶可使每毫升藻酸钠溶液的A235nm每分钟增加1.0
想查看有关 复合碳水化合物分析酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Elizabeth M Parrish et al.
Reproduction (Cambridge, England), 142(2), 309-318 (2011-05-26)
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.
Solaleh Khoramian Tusi et al.
Biomaterials, 32(23), 5438-5458 (2011-05-06)
Oxidative stress is a major component of harmful cascades activated in neurodegenerative disorders. We sought to elucidate possible effects of alginate oligosaccharide (AOS) on H(2)O(2)-induced cell death and to determine the underlying molecular mechanisms in neuron-like PC12 cells. We found
Lena C E Lundqvist et al.
Carbohydrate research, 352, 44-50 (2012-03-21)
The gene coding for an alginate lyase from the marine bacteria Pseudomonas alginovora X017 was cloned and heterologously expressed in Escherichia coli strains. The protein was produced in inclusion bodies and the active form was obtained by applying a refolding
Adam J Wargacki et al.
Science (New York, N.Y.), 335(6066), 308-313 (2012-01-24)
Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio
Yuya Morimoto et al.
APL bioengineering, 4(2), 026101-026101 (2020-04-09)
Biohybrid robots composed of biological and synthetic components have been introduced to reconstruct biological functions in mechanical systems and obtain better understanding of biological designs. For example, biohybrid robots powered by skeletal muscle tissue have already succeeded in performing various
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持