A1935
光敏生物素 乙酸盐
For labeling DNA probes for hybridizations
别名:
N-(4-叠氮-2-硝基苯基)-N′-(3-生物素基氨丙基)-N′-甲基-1,3-丙二胺 乙酸盐, 生物素{3-[3-(4-叠氮-2-硝基苯胺)-N-甲基丙氨基]丙酰胺} 乙酸盐
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关于此项目
经验公式(希尔记法):
C23H35N9O4S · C2H4O2
化学文摘社编号:
分子量:
593.70
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.52
等级
Molecular Biology
质量水平
方案
≥95%
表单
film or powder
溶解性
H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
储存温度
−20°C
SMILES字符串
CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
InChI
1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
InChI key
FFBLNTOMOSLSQM-AYEYRVMASA-N
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一般描述
光生物素乙酸盐由生物素组成,通过带电荷连接臂,与光反应性芳基叠氮化物基团结合。
应用
光生物素乙酸盐用于标记DNA探针,进行DNA−DNA杂交 确定基因组相关性。
生化/生理作用
光生物素乙酸盐能够标记单链DNA和线性或超螺旋双链DNA。
光生物素是可光活化的生物素类似物,主要用于标记DNA、RNA和蛋白。光生物素制备的探针,可检测低至0.5 pg的目标DNA。
免责声明
光敏。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Preparation and uses of photobiotin
Methods in Enzymology, 184, 588-600 (1990)
Chang Soo Lee et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1775-1779 (2007-08-09)
A novel thiosulfate-oxidizing bacterium, designated strain LCS2(T), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. The strain was found to comprise Gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on R2A agar. The strain
Rhodanobacter thiooxydans sp. nov., isolated from a biofilm on sulfur particles used in an autotrophic denitrification process
Lee CS, et al.
International Journal of Systematic and Evolutionary Microbiology, 57(8), 1775-1779 (2007)
Kwang Kyu Kim et al.
International journal of systematic and evolutionary microbiology, 55(Pt 5), 2075-2079 (2005-09-17)
A novel xylan-degrading bacterium, S3-E(T), was isolated from the biofilm of a membrane bioreactor. The cells of this strain were Gram-positive, non-motile, non-spore-forming rods, produced primary branches and formed yellow colonies on nutrient agar. The strain had chemotaxonomic markers that
Long Jin et al.
International journal of systematic and evolutionary microbiology, 57(Pt 8), 1876-1880 (2007-08-09)
Two novel bacteria, strains TR7-09(T) and P2-12-1, were isolated from samples of compost and river sediment, respectively. The strains comprised Gram-negative, motile, non-spore-forming rods, produced creamy white colonies on R2A agar, contained Q-8 as the predominant ubiquinone, contained iso-15 :
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