Anti-Apolipoprotein J antibody produced in goat

Apolipoprotein J is a ubiquitously expressed glycoprotein that is involved in the differentiation and proliferation of cells. Apolipoprotein J in conjunction with apolipoprotein E may affect the onset of amyloid-beta deposition, and also may be involved in the local toxicity associated with these deposits. Hence, apolipoprotein J may function as a cerebrospinal fluid marker for Alzheimer′s disease.
Goat anti-apolipoprotein J antibody recognizes human apolipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apolipoproteins.

Apolipoprotein Type J isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Goat anti-apolipoprotein J antibody can be used for immunohistochemistry applications using formalin-fixed, paraffin-embedded sections at a concentration ranging from 1:50-1:200. The antibody is also suitable for use in ELISA.

HEK293 cells were transfected with either an expression vector to over expression Clusterin or siRNA to knock down expression of Clusterin. Clusterin levels were monitored by western blot using goat anti-Clusterin antibody at 1:1000.

Solution in 0.1 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, and 0.01% sodium azide as preservative.