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线性分子式:
(NH4)2SO4
化学文摘社编号:
分子量:
132.14
PubChem Substance ID:
eCl@ss:
38030408
UNSPSC Code:
12352314
NACRES:
NA.31
EC Number:
231-984-1
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 0.667 g/mL
产品名称
硫酸铵, Molecular Biology, ≥99.0%
InChI key
BFNBIHQBYMNNAN-UHFFFAOYSA-N
InChI
1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
SMILES string
N.N.OS(O)(=O)=O
grade
Molecular Biology
assay
≥99.0%
form
powder
technique(s)
affinity binding assay: suitable
protein purification: suitable
impurities
≤0.005% Insolubles
pH
5.0-6 (25 °C, 132 g/L)
mp
>280 °C (dec.) (lit.)
solubility
H2O: 0.667 g/mL
density
1.77 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm
cation traces
Cu: ≤5 ppm
Fe: ≤5 ppm
Zn: ≤5 ppm
heavy metals (as Pb): ≤2 ppm
absorption
≤0.025 at 280 at 1 M
≤0.03 at 260 at 1 M
suitability
suitable for flow cytometry
suitable for molecular biology
application(s)
metabolomics
microbiology
foreign activity
DNase, RNase, and protease, none detected
Quality Level
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Application
硫酸铵有助于细胞蛋白的快速沉淀。 硫酸铵可用于蛋白质的沉淀或分级,或抗体的纯化。它也用于核酸和蛋白质的晶体学分析。硫酸铵已用于
- 蛋白的′蓝银′ 胶体考马斯染液的制备。
- 沉淀培养上清液中的志贺样毒素(Shiga-like toxin)。
- 沉淀培养上清液中的病毒颗粒。
Features and Benefits
- 重金属(以铅计):≤2 ppm
- 不含 RNase/DNase和蛋白酶。
General description
硫酸铵是一种用途广泛的无机硫酸盐,在生化研究、分子生物学和细胞培养中具有至关重要的作用。它是纯化抗体和沉淀或分离蛋白质不可或缺的工具。此外,它有助于核酸和蛋白质的晶体学分析,还可用作合成实验室培养基中的氮补充剂。 我们的精品硫酸铵经过精心配制,不含 DNase、RNase 和蛋白酶,确保其适合尖端研究。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization
David S Powlson, Chris J Dawson
Soil use and management, 38(1), 622-634 (2022)
Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry
Wayan Suardana et al.
Asian Pacific Journal of Cancer Prevention, 23-10, 3247-3252 (2022)
Protein precipitation using ammonium sulfate
P Wingfield
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 3 (2001)
William P Heal et al.
Nature protocols, 7(1), 105-117 (2011-12-24)
A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme
Mohd Baasir Gaisawat et al.
Microorganisms, 8(1) (2020-01-08)
Clostridium (C.) difficile-infection (CDI), a nosocomial gastrointestinal disorder, is of growing concern due to its rapid rise in recent years. Antibiotic therapy of CDI is associated with disrupted metabolic function and altered gut microbiota. The use of probiotics as an
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