A4605
抗iASPP抗体，小鼠单克隆抗体
clone LXO49.3, purified from hybridoma cell culture
别名:
p53家族凋亡刺激蛋白的抗抑制成员, 抗-RAI, 抗-Rel相关抑制剂
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关于此项目
Conjugate:
unconjugated
Clone:
LXO49.3, monoclonal
Application:
ARR, ICC, IP, WB
Citations:
11
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LXO49.3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
mouse, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 0.1-0.2 μg/mL using total cell extract of human osteogenic sarcoma (U-2-OS)
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PPP1R13L(10848)
mouse ... Ppp1r13l(333654)
General description
iASPP是p53（ASPP）家族的凋亡刺激蛋白的抑制成员。它是一种癌蛋白，与Ras，E1A和E7结合以转化细胞。
单克隆抗iASPP（小鼠IgG1同种型）衍生自杂交瘤LXO49，该杂交瘤是通过小鼠骨髓瘤细胞（SP2/0细胞）和来自用编码人iASPP残基的重组蛋白免疫的BALB/c小鼠的脾细胞融合产生的。ASPP蛋白家族包含三个成员，ASSP1、ASSP2和iASPP。iASPP也称为REL相关抑制剂（RAI）。iASPP以100 kDa形式和70 kDa形式（iASPP/Rai）存在。它在细胞核和细胞质中都发现，而iASPP/RAI主要是胞质的。PPP1R13L（蛋白磷酸酶1调节亚基13样）编码位于人染色体19q13上的iASPP。
Application
小鼠单克隆抗iASPP抗体可用于免疫印迹（0.1-0.2 μg/mL，使用人成骨肉瘤的细胞总提取物）、微阵列、免疫沉淀和免疫细胞化学。
小鼠抗iASPP单克隆抗体还可用于：
- 免疫印迹
- 免疫细胞化学
- 免疫沉淀
Biochem/physiol Actions
PPP1R13L（蛋白磷酸酶1调节亚基13样）/iASPP抑制p53介导的细胞死亡。它相互作用并抑制NFkB p65 RelA。它可能作为膀胱癌（BC）的治疗靶标。它调节细胞增殖和凋亡。iASPP在乳腺癌和急性白血病中起关键作用。
Physical form
溶于含有15 mM叠氮化钠的0.01 M磷酸盐缓冲液中。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity
Kramer D, et al.
Cell Death & Disease, 6(2), e1634-e1634 (2015)
The miR-124-p63 feedback loop modulates colorectal cancer growth
Liu K, et al.
Oncotarget, 8(17), 29101-29101 (2017)
Wenjie Ge et al.
Cancer cell, 32(5), 561-573 (2017-10-17)
Reactive oxygen species (ROS) have emerged as important signaling molecules that play crucial roles in carcinogenesis and cytotoxic responses. Nrf2 is the master regulator of ROS balance. Thus, uncovering mechanisms of Nrf2 regulation is important for the development of alternative
Ariel M Wilson et al.
PloS one, 9(4), e94175-e94175 (2014-04-10)
The transcription factor p53 mediates the apoptosis of post-mitotic neurons exposed to a wide range of stress stimuli. The apoptotic activity of p53 is tightly regulated by the apoptosis-stimulating proteins of p53 (ASPP) family members: ASPP1, ASPP2 and iASPP. We
Anissa Chikh et al.
The EMBO journal, 30(20), 4261-4273 (2011-09-08)
iASPP, an inhibitory member of the ASPP (apoptosis stimulating protein of p53) family, is an evolutionarily conserved inhibitor of p53 which is frequently upregulated in human cancers. However, little is known about the role of iASPP under physiological conditions. Here
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