产品名称
琼脂, Type E, suitable for plant cell culture
InChI key
GYYDPBCUIJTIBM-DYOGSRDZSA-N
InChI
1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
biological source
algae (Rhodophyceae)
type
Type E
form
powder
technique(s)
cell culture | plant: suitable
transition temp
transition temp 32-38 °C (1.5%)
application(s)
agriculture
storage temp.
room temp
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Application
Agar has been used as reagent for the following studies:
- Plant cell culture studies.
- As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
- For the solidification of culture medium of cork oak somatic embryos.
- to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants
Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.
General description
Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Pere Puigderrajols et al.
Journal of experimental botany, 53(373), 1445-1452 (2002-05-22)
The timing and tissue localization of small heat shock proteins (sHSPs) during cork oak somatic embryo development was investigated under normal growing culture conditions and in response to stress. Western blot analyses using polyclonal antibodies raised against cork oak recombinant
Melissa S LeBlanc et al.
Journal of biotechnology, 163(1), 1-9 (2012-10-31)
Arsenic is a toxic metalloid and recognized carcinogen. Arsenate and arsenite are the most common arsenic species available for uptake by plants. As an inorganic phosphate (Pi) analog, arsenate is acquired by plant roots through endogenous Pi transport systems. Inside
Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal (loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana
Uraguchi S, et al.
Frontiers in Plant Science, 11, 503-503 (2020)
Human bone marrow colony growth in agar-gel.
B L Pike et al.
Journal of cellular physiology, 76(1), 77-84 (1970-08-01)
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.
I MACPHERSON et al.
Virology, 23, 291-294 (1964-06-01)
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