生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 45 kDa
种属反应性
mouse, human, rat
技术
indirect immunofluorescence: 1:100 using mouse fibroblasts NIH3T3 cell line
microarray: suitable
western blot: 1:1,000 using rat brain cytosol extract or whole cell extract of human epidermoid carcinoma A431 cell line.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ACTR1A(10121)
mouse ... Actr1a(54130)
rat ... Actr1a(294010)
一般描述
The actin-related protein Arp1α (centractin, actin-RPV), a member of the superfamily of actin-related proteins (Arp), is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. It has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores.
免疫原
synthetic peptide corresponding to the mid-region of human Arp1α/centractin (amino acids 222-240). This sequence is identical in mouse and dog Arp1α/centractin, highly conserved (89% identity) in the α-centractin isoform, and not found in known actin isoforms.
应用
Anti-Arp1α/Centractin antibody produced in rabbit has been used in immunoblotting and immunofluorescence staining.
生化/生理作用
Arp1α or Centractin is an actin-related, ubiquitious protein that forms a part of the dynactin complex and is associated with centrosomes. Studies have reported that centractin interacts with spectrin and subsequently provides a link for dynactin to associate with organelles in cells.
The actin-related proteins (Arp)1α/dynactin complex regulates dyne mediated vesicle movement on microtubules as well as spindle assembly and cell division. Arp1α overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of Arp1α/dynactin complex in progression through the prometaphase of mitosis. Expression of mutant Arp1a protein has no effect on mitotic cells, but results in microtubule disruption in interphase cells, by destabilizing the interaction between Arp1a and components of the dynactin complex and nuclear mitotic apparatus protein (NuMA).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Regulation of spindle integrity and mitotic fidelity by BCCIP
Huhn S, et al.
Oncogene, 36(33), 4750-4750 (2017)
Antimitotic effect of the retinoid 4-oxo-fenretinide through inhibition of tubulin polymerization: a novel mechanism of retinoid growth-inhibitory activity
Appierto V, et al.
Molecular Cancer Therapeutics, 8(12), 3360-3368 (2009)
S W Clark et al.
Nature, 359(6392), 246-250 (1992-09-17)
Actin is one of the most ubiquitous, abundant and well-conserved proteins of eukaryotes, participating in many crucial cellular processes including the maintenance of cell shape, motility and cell division. Actins from the most divergent sources still share amino-acid identities in
S C Huhn et al.
Oncogene, 36(33), 4750-4766 (2017-04-11)
Centrosomes together with the mitotic spindle ensure the faithful distribution of chromosomes between daughter cells, and spindle orientation is a major determinant of cell fate during tissue regeneration. Spindle defects are not only an impetus of chromosome instability but are
E A Holleran et al.
The Journal of cell biology, 135(6 Pt 2), 1815-1829 (1996-12-01)
Centractin (Arp1), an actin-related protein, is a component of the dynactin complex. To investigate potential functions of the protein, we used transient transfections to overexpress centractin in mammalian cells. We observed that the overexpressed polypeptide formed filamentous structures that were
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