A6535
三磷酸腺苷双磷酸酶 来源于马铃薯
ATPase ≥200 units/mg protein, lyophilized powder
别名:
腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
应用
三磷酸腺苷双磷酸酶用于水解核苷三磷酸和二磷酸。水解有机二磷酸和三磷酸的最佳pH为6，水解无机底物的最佳pH为5.1。Sigma的三磷酸腺苷双磷酸酶已用于血小板聚集的抑制研究。
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+Pi → AMP+2Pi。
反应：ATP → ADP+Pi → AMP+2Pi。
生化/生理作用
三磷酸腺苷双磷酸酶存在于所有真核生物和部分原核生物中。 马铃薯三磷酸腺苷双磷酸酶在调节生长和发育中发挥着至关重要的作用。 三磷酸腺苷双磷酸酶在神经刺激后可作为神经递质参与突触ATP的失活，也参与抑制ADP诱导的血小板聚集，防止血栓形成。 其活性需要二价金属离子，在5 mM钙离子存在下观察到最佳活性。
包装
按 ATP 酶单位销售。
外形
部分纯化的冻干粉，含琥珀酸钾缓冲盐。
制备说明
来源于红皮土豆
其他说明
在pH 6.5、30℃条件下，一单位酶每分钟可从ATP或ADP释放1.0 μmM无机磷酸盐。
该等级具有低 ATP 酶/ADP 酶比率。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Serena Sirigu et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(47), E7448-E7455 (2016-11-07)
Direct inhibition of smooth muscle myosin (SMM) is a potential means to treat hypercontractile smooth muscle diseases. The selective inhibitor CK-2018571 prevents strong binding to actin and promotes muscle relaxation in vitro and in vivo. The crystal structure of the
Yiying Cai et al.
Microorganisms, 8(10) (2020-10-02)
Traditional in vitro time-kill studies (TKSs) require viable plating, which is tedious and time-consuming. We used ATP bioluminescence, with the removal of extracellular ATP (EC-ATP), as a surrogate for viable plating in TKSs against carbapenem-resistant Gram-negative bacteria (CR-GNB). Twenty-four-hour TKSs
Alexandra L Chang-Graham et al.
Science (New York, N.Y.), 370(6519) (2020-11-21)
Rotavirus causes severe diarrheal disease in children by broadly dysregulating intestinal homeostasis. However, the underlying mechanism(s) of rotavirus-induced dysregulation remains unclear. We found that rotavirus-infected cells produce paracrine signals that manifested as intercellular calcium waves (ICWs), observed in cell lines
Agnete Kirkeby et al.
Thrombosis and haemostasis, 99(4), 720-728 (2008-04-09)
The haematopoietic hormone erythropoietin (EPO) has neuroprotective properties and is currently being explored for treatment of stroke and other neurological disorders. Short-term, high-dose treatment with EPO seems to improve neurological function of stroke patients but may be associated with increased
Properties of Two Apyrases from Solanum tuberosum
Kettlun, A. et al.
Phytochemistry, 21(3), 551-558 (1982)
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