A6691
酰胺酶 来源于绿脓假单胞菌
recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)
别名:
丙烯酰胺酰胺水解酶, 酰基转移酶
产品名称
酰胺酶 来源于绿脓假单胞菌, recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)
recombinant
expressed in E. coli
form
buffered aqueous glycerol solution
hydroxamate transferase activity
≥200 units/mg protein (biuret)
concentration
14 mg/mL
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
Pseudomonas aeruginosa PAO1 ... PA4163(880181)
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Application
来自铜绿假单胞菌的酰胺酶已被用于测试其水解曲毒素A的能力。
这些水解酶对于生物技术的重要性正在迅速增加，因为它们的潜在应用遍及化学和制药行业以及生物修复。 固定化酰胺酶可被有效地用于从丙烯酰胺产生丙烯酸，从而将有毒的环境污染物转化为广泛使用的工业原材料。酰胺酶是治疗人类免疫缺陷病毒和疟疾的一种潜在方法。 它们可用于消除废水中的金属离子。
Biochem/physiol Actions
来自铜绿假单胞菌的酰胺酶是一种6 × 38-kDa的酶，可催化小范围短脂族酰胺的水解。 每种酰胺酶单体都是由一种带有额外81个残基长C末端区域的球形四层αββα三明治结构域所组成的。
Other Notes
在pH 7.2、37 °C下，一单位可在每分钟内将1.0 μmol的乙酰胺和羟胺转化为乙酰氧肟酸酯和氨。
Physical form
含有7 mM 2-巯基乙醇和磷酸盐缓冲液的50％甘油溶液
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis
Pacheco R, Karmali A
Biocatalysis and Biotransformation, 27(5/6), 367-376 (2009)
Jorge Andrade et al.
The Journal of biological chemistry, 282(27), 19598-19605 (2007-04-20)
Microbial amidases belong to the thiol nitrilases family and have potential biotechnological applications in chemical and pharmaceutical industries as well as in bioremediation. The amidase from Pseudomonas aeruginosa isa6 x 38-kDa enzyme that catalyzes the hydrolysis of a small range
Butyramide-utilizing mutants of Pseudomonas aeruginosa 8602 which produce an amidase with altered substrate specificity.
J E Brown et al.
Journal of general microbiology, 57(2), 273-285 (1969-08-01)
Arthur J L Cooper et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 163(1), 108-120 (2012-05-23)
Many cancer cells have a strong requirement for glutamine. As an aid for understanding this phenomenon the (18)F-labeled 2S,4R stereoisomer of 4-fluoroglutamine [(2S,4R)4-FGln] was previously developed for in vivo positron emission tomography (PET). In the present work, comparative enzymological studies
Desirée C Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(45), E1052-E1060 (2011-10-19)
ATP-binding cassette transporters are ubiquitous membrane protein complexes that move substrates across membranes. They do so using ATP-induced conformational changes in their nucleotide-binding domains to alter the conformation of the transport cavity formed by their transmembrane domains. In Escherichia coli
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