A6691
酰胺酶 来源于绿脓假单胞菌
recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)
别名:
丙烯酰胺酰胺水解酶, 酰基转移酶
重组
expressed in E. coli
表单
buffered aqueous glycerol solution
hydroxamate transferase activity
≥200 units/mg protein (biuret)
浓度
14 mg/mL
UniProt登记号
储存温度
−20°C
基因信息
Pseudomonas aeruginosa PAO1 ... PA4163(880181)
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应用
来自铜绿假单胞菌的酰胺酶已被用于测试其水解曲毒素A的能力。
这些水解酶对于生物技术的重要性正在迅速增加，因为它们的潜在应用遍及化学和制药行业以及生物修复。 固定化酰胺酶可被有效地用于从丙烯酰胺产生丙烯酸，从而将有毒的环境污染物转化为广泛使用的工业原材料。酰胺酶是治疗人类免疫缺陷病毒和疟疾的一种潜在方法。 它们可用于消除废水中的金属离子。
生化/生理作用
来自铜绿假单胞菌的酰胺酶是一种6 × 38-kDa的酶，可催化小范围短脂族酰胺的水解。 每种酰胺酶单体都是由一种带有额外81个残基长C末端区域的球形四层αββα三明治结构域所组成的。
外形
含有7 mM 2-巯基乙醇和磷酸盐缓冲液的50％甘油溶液
其他说明
在pH 7.2、37 °C下，一单位可在每分钟内将1.0 μmol的乙酰胺和羟胺转化为乙酰氧肟酸酯和氨。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
Jorge Andrade et al.
The Journal of biological chemistry, 282(27), 19598-19605 (2007-04-20)
Microbial amidases belong to the thiol nitrilases family and have potential biotechnological applications in chemical and pharmaceutical industries as well as in bioremediation. The amidase from Pseudomonas aeruginosa isa6 x 38-kDa enzyme that catalyzes the hydrolysis of a small range
Substrate interaction with recombinant amidase from Pseudomonas aeruginosa during biocatalysis
Pacheco R, Karmali A
Biocatalysis and Biotransformation, 27(5/6), 367-376 (2009)
Butyramide-utilizing mutants of Pseudomonas aeruginosa 8602 which produce an amidase with altered substrate specificity.
J E Brown et al.
Journal of general microbiology, 57(2), 273-285 (1969-08-01)
Mo-Fei Li et al.
Fish & shellfish immunology, 32(2), 322-330 (2011-12-08)
Peptidoglycan recognition proteins (PGRPs) are a family of innate immune molecules that recognize bacterial peptidoglycan. PGRPs are highly conserved in invertebrates and vertebrates including fish. However, the biological function of teleost PGRP remains largely uninvestigated. In this study, we identified
Sebastian Zoll et al.
Journal of bacteriology, 194(15), 3789-3802 (2012-05-23)
The bifunctional major autolysin Atl plays a key role in staphylococcal cell separation. Processing of Atl yields catalytically active amidase (AM) and glucosaminidase (GL) domains that are each fused to repeating units. The two repeats of AM (R1 and R2)
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