生物来源
goat
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
种属反应性
rabbit
请勿与下列物质发生反应
human
技术
direct ELISA: 1:1,000
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat anti-rabbit IgG-alkaline phosphatase antibody binds all rabbit Igs and does not cross react with human serum proteins.
Goat anti-rabbit IgG-alkaline phosphatase antibody binds all rabbit Igs and does not cross react with human serum proteins.
免疫原
purified rabbit IgG
应用
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用,使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液(Ph 9.6)中以1:5000稀释的二次稀释剂。
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Goat anti-rabbit IgG-alkaline phosphatase antibody has been used for immunocytochemistry and western blotting (1:1,000) applications. The product may also be used for direct ELISA at 1:1,000.
Western blot analysis of TRAP expression after viral transduction in Sf9 cells was performed using alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary at 1:500.
外形
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
其他说明
Antibody adsorbed with human serum proteins
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
José Alage Baldé et al.
Journal of plant physiology, 163(1), 19-25 (2005-12-20)
Fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (Cucumis melo L.). In this work the presence of a class III chitinase was examined by immunological techniques in the root and stem
Shaoping Lu et al.
Plant biotechnology journal, 11(3), 380-389 (2013-01-03)
The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of
J L Ried et al.
Plant physiology, 93(2), 662-667 (1990-06-01)
Germination of embryonic axes from dormant grain is inhibited by low concentrations of abscisic acid (ABA) compared with axes from nondormant grain. Incubation of dormant grain axes in 0.05 to 50 micromolar ABA caused the prolonged synthesis of a set
William T Hay et al.
Journal of plant physiology, 212, 58-68 (2017-03-09)
Soybean C3 photosynthesis can suffer a severe loss in efficiency due to photorespiration and the lack of a carbon concentrating mechanism (CCM) such as those present in other plant species or cyanobacteria. Transgenic soybean (Glycine max cv. Thorne) plants constitutively
Ashley K Spence et al.
Journal of experimental botany, 65(13), 3737-3747 (2014-06-25)
Miscanthus × giganteus is exceptional among C4 plants in its ability to acclimate to chilling (≤14 °C) and maintain a high photosynthetic capacity, in sharp contrast to maize, leading to very high productivity even in cool temperate climates. To identify
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