ArrayHyb^™ Hybridization Buffer

In response to the need for a hybridization buffer with high signal-to-noise ratios in fluorescent microarray analysis, Sigma has developed ArrayHyb^™ and ArrayHyb^™ LowTemp Microarray Hybridization Buffers.
ArrayHyb and ArrayHyb LowTemp are optimized, low-viscosity, ready-to-use hybridization buffers designed for use in gene expression analysis with cDNA or oligonucleotide probes spotted onto glass slides. Hybridizations with fluorescently labeled nucleic acids consistently provide superior signal and minimal background compared to standard and other commercially available hybridization buffers. Both formulations increase the rate of hybridization and can provide signals for analytical purposes in as little as 2 hours. Approximately 60-80% of the maximum signal is obtained in six hours, with extended incubation times resulting in increased sensitivity without increased background.
ArrayHyb is designed to replace standard microarray hybridization buffers when hybridizing at temperatures greater than 60°C.
This flexibility of ArrayHyb buffers allows hybridizations to be tailored to meet your specific requirements. ArrayHyb buffers are not only flexible, but also versatile and may be used with PCR products, genomic DNA, or oligonucleotides probes arrayed on glass slides.

Sigma offers optimized, low-viscosity, ready-to-use hybridization buffers designed for use in gene expression analysis with probes spotted onto glass slides. ArrayHyb buffers may be used with PCR products, genomic DNA, or oligonucleotides probes arrayed on glass slides.

ArrayHyb is an optimized microarray hybridization buffer recommended for use when hybridizing at temperatures greater than 60 °C.

ArrayHyb is a trademark of Sigma-Aldrich Co. LLC