biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
clone
5F10, monoclonal
contains
15 mM sodium azide
species reactivity
baboon, feline, plant, rat, chimpanzee, monkey, eel, human, rabbit, chicken, canine, parasites
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:100 using extracts of human erythrocyte ghosts
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... ATP2A3(489)
rat ... Atp2a3(25391)
General description
Calcium ATPase is a transport protein that removes calcium from the cell after muscle contraction. There are two known types, PMCA which is found in the plasma membrane, and the sarcoplasmic reticulum specific protein SERCA.
Immunogen
human erythrocyte Ca2+-ATPase.
Biochem/physiol Actions
The antibody reacts specifically with human erythrocyte Ca2+ pump (Ca2+-ATPase, 138 kDa). It may also recognize additional bands at ~215, 160-180, 125, 95-107, 72, and 27 kDa representing products of aggregation or proteolysis of the pump. The antibody does not significantly affect the Ca2+ pump activity. The reactivity of the antibody has been mapped to an epitope within amino acid residues 724-783, the region proposed to be the hinge domain of the Ca2+-ATPase molecule. It cross-reacts with the Ca2+ pump of basolateral membrane of distal convoluted tubules of kidney, hepatocyte plasma membrane, intestine, placenta, endometrium, choroid plexus, Purkinje cells, and hen oviductal tissue using immunohistochemistry and immunoblotting. In human kidney, specific staining is found only along the basolateral membrane of the distal convoluted tubules, while glomeruli and other segments of the nephron do not stain. An additional application with this antibody is immunoaffinity purification of the Ca2+ pump.