生物来源
mouse
偶联物
unconjugated
抗体形式
ascites fluid
克隆
5F10, monoclonal
包含
15 mM sodium azide
种属反应性
baboon, feline, plant, rat, chimpanzee, monkey, eel, human, rabbit, chicken, canine, parasites
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:100 using extracts of human erythrocyte ghosts
同位素/亚型
IgG2a
运输
dry ice
储存温度
−20°C
基因信息
human ... ATP2A3(489)
rat ... Atp2a3(25391)
一般描述
Calcium ATPase is a transport protein that removes calcium from the cell after muscle contraction. There are two known types, PMCA which is found in the plasma membrane, and the sarcoplasmic reticulum specific protein SERCA.
免疫原
human erythrocyte Ca2+-ATPase.
生化/生理作用
The antibody reacts specifically with human erythrocyte Ca2+ pump (Ca2+-ATPase, 138 kDa). It may also recognize additional bands at ~215, 160-180, 125, 95-107, 72, and 27 kDa representing products of aggregation or proteolysis of the pump. The antibody does not significantly affect the Ca2+ pump activity. The reactivity of the antibody has been mapped to an epitope within amino acid residues 724-783, the region proposed to be the hinge domain of the Ca2+-ATPase molecule. It cross-reacts with the Ca2+ pump of basolateral membrane of distal convoluted tubules of kidney, hepatocyte plasma membrane, intestine, placenta, endometrium, choroid plexus, Purkinje cells, and hen oviductal tissue using immunohistochemistry and immunoblotting. In human kidney, specific staining is found only along the basolateral membrane of the distal convoluted tubules, while glomeruli and other segments of the nephron do not stain. An additional application with this antibody is immunoaffinity purification of the Ca2+ pump.
法规信息
新产品
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R H Wasserman et al.
Histochemistry, 96(5), 413-418 (1991-01-01)
The localization of a plasma membrane calcium pump in the oviduct of the laying hen was investigated by immunohistochemical techniques, utilizing a monoclonal antibody (5F10) produced against the human erythrocyte calcium pump. This antibody was shown to react with an
J L Borke et al.
The Journal of clinical investigation, 80(5), 1225-1231 (1987-11-01)
Human calcium transporting tissues were examined to determine whether they contained a protein similar to the Ca++-Mg++ adenosine triphosphatase (Ca++-Mg++ATPase) pump of the human erythrocyte membrane. Tissues were processed for immunoperoxidase staining using monoclonal antibodies against purified Ca++-Mg++ATPase. In human
J L Borke et al.
Brain research, 489(2), 355-360 (1989-06-12)
A major unanswered question in central nervous system physiology concerns the mechanism by which cerebrospinal fluid (CSF) Ca2+ homeostasis is maintained in the face of hypo- or hypercalcemia. To address this question, we sought and found a protein of Mr
J L Borke et al.
Pflugers Archiv : European journal of physiology, 417(1), 120-122 (1990-09-01)
We used a monoclonal antibody (5F10) specific for the human erythrocyte plasma membrane Ca(++)-pump to demonstrate the presence and distribution of Ca(++)-pump epitopes in rat intestine. In paraffin embedded tissue sections, antibody 5F10 binds to epitopes in the basolateral membranes
J L Borke et al.
The American journal of physiology, 257(5 Pt 2), F842-F849 (1989-11-01)
In an effort to extend our studies on Ca2+ pumps to animal models, we developed a new monoclonal antibody (5F10) prepared against the human erythrocyte Ca2+-Mg2+-adenosinetriphosphatase (ATPase) that recognizes a protein of approximately 140 kDa in rat kidney homogenates. Enzyme-linked
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